Composite

Part:BBa_K4343069:Design

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)


pUC-HUH-PaDes17(LEU)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2102
    Illegal XhoI site found at 667
    Illegal XhoI site found at 696
    Illegal XhoI site found at 1844
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1239
    Illegal AgeI site found at 1667
    Illegal AgeI site found at 2769
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1242
    Illegal BsaI site found at 1670
    Illegal BsaI site found at 2772
    Illegal BsaI.rc site found at 1236
    Illegal BsaI.rc site found at 1664
    Illegal BsaI.rc site found at 2766
    Illegal BsaI.rc site found at 3036
    Illegal SapI site found at 1078


Design Notes

NONE


Source

from Pythium aphanidermatum

References